Cross-group report

3 summarized samples · GRPs · 2026/9/30 09:50:40

Full coverage: 3 of 3 selected samples and 2 of 2 selected groups are summarized.
Samples3
Events540,000
Populations2
QC reviewed0

Report composition

OrderSectionTitleCaption
1 Gating hierarchy Gating hierarchy no
2 Population stats Population stats no
3 Multi-plot layout Multi-plot layout no
4 QC table QC table no
5 Workflow trail Workflow trail no
6 Text note Methods note yes

Statistics scope

SectionStatistic scopeMeaning
Cohort population summaryselected GRP samplesAll summarized sample-level gate statistics pooled across the report scope.
Group comparisonselected GRPsAlways grouped by the user-selected GRP names, independent of the control comparison unit.
Control comparisonMetadata: condition% Parent; control Control.
Prism exportMetadata: conditionUses the same comparison setup as Control comparison; tables are 2 populations.
Appendix/detailssample rows within selected GRPsSample-level rows are audit detail and should reconcile to GRP summaries when the report is complete.

Shared cross-group tile template

Shared cross-group tile template Each selected group renders the same tile set in the same order so the report stays comparable.
Tile 1 Representative layout Same multi-plot tile order for every selected group.
Tile 2 Population hierarchy Shared gate and population identity across groups.
Tile 3 Population summary Matched summary rows for %Parent, range, and CV.
Tile 4 Control comparison Shared contrast tables and deltas across the same populations.
Tile 5 QC and provenance Consistent QC, metadata, and workflow context per group.

Gating hierarchy

Gating strategy tier images

All Events tier plot
All Events root · 240,000 events

Population summary

PopulationnMean %ParentRangeCVMFI: DAPI-AMedian: DAPI-A
Illustrative cells gate383.16%83.12-83.18%0.00......
Illustrative CD3+ gate380.26%80.14-80.43%0.00......

Group comparison

PopulationGroupnMean %ParentRangeΔ groupsMFI: DAPI-AMedian: DAPI-A
Illustrative CD3+ gateControl180.14%80.14-80.14%0.00......
Illustrative CD3+ gateTreated280.32%80.20-80.43%0.00......
Illustrative cells gateControl183.18%83.18-83.18%0.00......
Illustrative cells gateTreated283.15%83.12-83.18%0.00......

Control comparison

Illustrative CD3+ gate +0.18% Treated vs Control · 1.00x · d=n/a · p=n/a
Illustrative cells gate -0.02% Treated vs Control · 1.00x · d=n/a · p=n/a
Population Comparison Control mean Compare mean Δ % Parent SEM Cohen's d p / q Fold n
Illustrative CD3+ gate Treated vs Control 80.14% 80.32% +0.18% 0.00% / 0.11% - - 1.00x 1/2
Illustrative cells gate Treated vs Control 83.18% 83.15% -0.02% 0.00% / 0.03% - - 1.00x 1/2

Group detail tiles

Control 1 sample · 180,000 events · 2 populations
Expand detail tiles
Representative sampleDemo cohort 1.fcs
Tile setShared
Populations2
Representative layoutPopulation hierarchyPopulation summaryControl comparisonQC and provenance

Representative layout

Demo cohort 1.fcs

Population summary

PopulationnMean %ParentRangeCV
Illustrative cells gate 1 83.18% 83.18-83.18% 0.00
Illustrative CD3+ gate 1 80.14% 80.14-80.14% 0.00
Treated 2 samples · 360,000 events · 2 populations
Expand detail tiles
Representative sampleDemo cohort 2.fcs
Tile setShared
Populations2
Representative layoutPopulation hierarchyPopulation summaryControl comparisonQC and provenance

Representative layout

Demo cohort 2.fcs

Population summary

PopulationnMean %ParentRangeCV
Illustrative cells gate 2 83.15% 83.12-83.18% 0.00
Illustrative CD3+ gate 2 80.32% 80.20-80.43% 0.00

QC status

SampleQCReview
Demo cohort 1.fcsnot runreported
Demo cohort 2.fcsnot runreported
Demo cohort 3.fcsnot runreported

Figure notes

Methods note

Capture methods, caveats, or audit notes for the appendix.

Sample details

SampleGroupPopulationTypeEvents% Parent% TotalMFI: DAPI-AMedian: DAPI-A
Demo cohort 1.fcsControlAll Eventsroot180,000100.00%100.00%......
Demo cohort 1.fcsControlIllustrative cells gaterectangle149,72283.18%83.18%......
Demo cohort 1.fcsControlIllustrative CD3+ gaterectangle119,98080.14%66.66%......
Demo cohort 2.fcsTreatedAll Eventsroot180,000100.00%100.00%......
Demo cohort 2.fcsTreatedIllustrative cells gaterectangle149,73283.18%83.18%......
Demo cohort 2.fcsTreatedIllustrative CD3+ gaterectangle120,08680.20%66.71%......
Demo cohort 3.fcsTreatedAll Eventsroot180,000100.00%100.00%......
Demo cohort 3.fcsTreatedIllustrative cells gaterectangle149,62483.12%83.12%......
Demo cohort 3.fcsTreatedIllustrative CD3+ gaterectangle120,34280.43%66.86%......

Metadata and workflow audit

Metadata

Recent workflow steps