{
  "schema": "flowapp.flowrepository-cell-cycle-preflight.v1",
  "generatedAt": "2026-09-16T14:12:24.172Z",
  "dnaChannel": {
    "parameter": "FL2-A (PE-A)",
    "backgroundControl": "Control no PI.fcs",
    "threshold": 33795.6015625,
    "rationale": "FL2-A is the PI DNA candidate in the source FCS: the selected no-PI control establishes the 99.5th-percentile background threshold. Exported reference files contain this signal only, normalized and labeled as linear PI-A."
  },
  "gatePolicy": "Broad morphology cleanup, FSC-A/FSC-H singlet selection, then PI-positive selection. FlowJo and FlowApp must both analyze only the exported single-channel linear PI-A FCS.",
  "rows": [
    {
      "source": "FR-FCM-Z55H",
      "sample": "Control 1 - Control.fcs",
      "sourceSha256": "b1b5a0ceac89daa7db650595da1e850273003a0c96b4ea375e5a9b5d0dd3896d",
      "rawEvents": 10000,
      "gatedEvents": 7907,
      "selectionRate": 0.7907,
      "dnaParameter": "PI-A",
      "isBackgroundControl": false,
      "eligibleForReference": true,
      "gate": {
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      "gateDescription": "FSC-A/SSC-A central 99%; log(FSC-A/FSC-H) median +/- 0.801; FL2-A (PE-A) > 33795.60",
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        "g2G1Ratio": 1.873709677419355
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      "status": "candidate"
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    {
      "source": "FR-FCM-Z55H",
      "sample": "Control 2.fcs",
      "sourceSha256": "0588429b4ee42e20e49d36f4615d8c253a98512c1e0dfe25a69f76aa201e295d",
      "rawEvents": 10000,
      "gatedEvents": 7347,
      "selectionRate": 0.7347,
      "dnaParameter": "PI-A",
      "isBackgroundControl": false,
      "eligibleForReference": true,
      "gate": {
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        "ratioCenter": 0.7765251263352062,
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      "gateDescription": "FSC-A/SSC-A central 99%; log(FSC-A/FSC-H) median +/- 0.404; FL2-A (PE-A) > 33795.60",
      "flowapp": {
        "model": "Independent DJF-style",
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        "g2G1Ratio": 1.892142857142857
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      "status": "candidate"
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    {
      "source": "FR-FCM-Z55H",
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      "rawEvents": 10000,
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      "selectionRate": 0.0021,
      "dnaParameter": "PI-A",
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      "gate": {
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      "gateDescription": "FSC-A/SSC-A central 99%; log(FSC-A/FSC-H) median +/- 0.348; FL2-A (PE-A) > 33795.60",
      "flowapp": null,
      "status": "background-control"
    },
    {
      "source": "FR-FCM-Z55H",
      "sample": "T317E PI 1.fcs",
      "sourceSha256": "2a4a3f84894b07ba0389fb68e8e16171ac47cb8b40a95b222de40a6985973476",
      "rawEvents": 10000,
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      "selectionRate": 0.7982,
      "dnaParameter": "PI-A",
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      "gate": {
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      "gateDescription": "FSC-A/SSC-A central 99%; log(FSC-A/FSC-H) median +/- 0.938; FL2-A (PE-A) > 33795.60",
      "flowapp": {
        "model": "Independent DJF-style",
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        "g0G1Percent": 38.514600001241575,
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      "status": "review-fit"
    },
    {
      "source": "FR-FCM-Z55H",
      "sample": "T317E PI 2.fcs",
      "sourceSha256": "5cbe7b9c60a67171b5cc0a293b4a5bbbfecf3553776c7b8fa020507b310e0b5c",
      "rawEvents": 10000,
      "gatedEvents": 6786,
      "selectionRate": 0.6786,
      "dnaParameter": "PI-A",
      "isBackgroundControl": false,
      "eligibleForReference": true,
      "gate": {
        "fscLow": 44048.69921875,
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      "gateDescription": "FSC-A/SSC-A central 99%; log(FSC-A/FSC-H) median +/- 0.399; FL2-A (PE-A) > 33795.60",
      "flowapp": {
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      "status": "review-fit"
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    {
      "source": "FR-FCM-Z55H",
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      "rawEvents": 10000,
      "gatedEvents": 6837,
      "selectionRate": 0.6837,
      "dnaParameter": "PI-A",
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      "eligibleForReference": true,
      "gate": {
        "fscLow": 51021.80078125,
        "fscHigh": 13196603,
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      "gateDescription": "FSC-A/SSC-A central 99%; log(FSC-A/FSC-H) median +/- 0.463; FL2-A (PE-A) > 33795.60",
      "flowapp": {
        "model": "Independent DJF-style",
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      "status": "review-fit"
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    {
      "source": "FR-FCM-Z55H",
      "sample": "T33 T317 E 2.fcs",
      "sourceSha256": "597aa01a1e49b73e15cb5ba61e4ec13b16e9bc283a25b18f5dc5c87573c4ac6d",
      "rawEvents": 10000,
      "gatedEvents": 7112,
      "selectionRate": 0.7112,
      "dnaParameter": "PI-A",
      "isBackgroundControl": false,
      "eligibleForReference": true,
      "gate": {
        "fscLow": 46266.6015625,
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        "sscLow": 7374.10009765625,
        "sscHigh": 10527525,
        "ratioCenter": 0.7815593487985647,
        "ratioMad": 0.1461332554120811,
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        "dnaBackgroundLimit": 33795.6015625
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      "gateDescription": "FSC-A/SSC-A central 99%; log(FSC-A/FSC-H) median +/- 0.585; FL2-A (PE-A) > 33795.60",
      "flowapp": {
        "model": "Independent DJF-style",
        "fitR2": 0.33797553432855487,
        "g0G1Percent": 0.0061178715921842005,
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        "g2G1Ratio": 2.0005172413793106
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      "status": "review-fit"
    },
    {
      "source": "FR-FCM-Z55H",
      "sample": "T33E 1.fcs",
      "sourceSha256": "37088041acff031e7d1c5683e21c61e7e95f3e6603dcd6b36c15801d71f19d1d",
      "rawEvents": 10000,
      "gatedEvents": 7292,
      "selectionRate": 0.7292,
      "dnaParameter": "PI-A",
      "isBackgroundControl": false,
      "eligibleForReference": true,
      "gate": {
        "fscLow": 48129.3984375,
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        "dnaBackgroundLimit": 33795.6015625
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      "gateDescription": "FSC-A/SSC-A central 99%; log(FSC-A/FSC-H) median +/- 0.422; FL2-A (PE-A) > 33795.60",
      "flowapp": {
        "model": "Independent DJF-style",
        "fitR2": 0.7921223254569055,
        "g0G1Percent": 29.723717156051695,
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        "g2G1Ratio": 1.851326530612245
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      "status": "candidate"
    },
    {
      "source": "FR-FCM-Z55H",
      "sample": "T33E 2.fcs",
      "sourceSha256": "07d3702cfd19e6e0197b4b37ba1ac7ac9fe6da874f9d294c65a688aafd792b75",
      "rawEvents": 10000,
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      "eligibleForReference": true,
      "gate": {
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        "dnaBackgroundLimit": 33795.6015625
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      "gateDescription": "FSC-A/SSC-A central 99%; log(FSC-A/FSC-H) median +/- 0.324; FL2-A (PE-A) > 33795.60",
      "flowapp": {
        "model": "Independent DJF-style",
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        "g0G1Percent": 48.41818703723374,
        "sPercent": 51.579927383452315,
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        "g2G1Ratio": 2.0865463917525773
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      "status": "review-fit"
    },
    {
      "source": "FR-FCM-Z55H",
      "sample": "WT no PI.fcs",
      "sourceSha256": "ab092e84e5501d7dd400c59d9c5f50425f0d73b08b95a78c7c18d1055d5f484b",
      "rawEvents": 10000,
      "gatedEvents": 3173,
      "selectionRate": 0.3173,
      "dnaParameter": "PI-A",
      "isBackgroundControl": true,
      "eligibleForReference": false,
      "gate": {
        "fscLow": 54331.6015625,
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        "dnaBackgroundLimit": 33795.6015625
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      "gateDescription": "FSC-A/SSC-A central 99%; log(FSC-A/FSC-H) median +/- 0.331; FL2-A (PE-A) > 33795.60",
      "flowapp": null,
      "status": "background-control"
    },
    {
      "source": "FR-FCM-Z55H",
      "sample": "WT1.fcs",
      "sourceSha256": "ef0c8eba55f4072a346d5150dd65ea862f315f6e37e5bc7b9ab4207e2eb61fb1",
      "rawEvents": 4378,
      "gatedEvents": 2166,
      "selectionRate": 0.49474645957058017,
      "dnaParameter": "PI-A",
      "isBackgroundControl": false,
      "eligibleForReference": true,
      "gate": {
        "fscLow": 36603.8984375,
        "fscHigh": 8713138,
        "sscLow": -8.699999809265137,
        "sscHigh": 6639172.5,
        "ratioCenter": 0.7030691523911807,
        "ratioMad": 0.16205117932644608,
        "ratioRadius": 0.6482047173057843,
        "dnaBackgroundLimit": 33795.6015625
      },
      "gateDescription": "FSC-A/SSC-A central 99%; log(FSC-A/FSC-H) median +/- 0.648; FL2-A (PE-A) > 33795.60",
      "flowapp": {
        "model": "Independent DJF-style",
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        "g2G1Ratio": 1.9149999999999998
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      "status": "review-fit"
    },
    {
      "source": "FR-FCM-Z55H",
      "sample": "WT2.fcs",
      "sourceSha256": "2a4379e682fadb5120396a110bc616e3beb988b9294f0c5d7f275a6ec24ff4e7",
      "rawEvents": 10000,
      "gatedEvents": 6730,
      "selectionRate": 0.673,
      "dnaParameter": "PI-A",
      "isBackgroundControl": false,
      "eligibleForReference": true,
      "gate": {
        "fscLow": 39793.1015625,
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        "sscLow": 4272,
        "sscHigh": 7739106.5,
        "ratioCenter": 0.7308988966594105,
        "ratioMad": 0.10462323815062646,
        "ratioRadius": 0.41849295260250585,
        "dnaBackgroundLimit": 33795.6015625
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      "gateDescription": "FSC-A/SSC-A central 99%; log(FSC-A/FSC-H) median +/- 0.418; FL2-A (PE-A) > 33795.60",
      "flowapp": {
        "model": "Independent DJF-style",
        "fitR2": 0.3575056093614517,
        "g0G1Percent": 0.005404709976197773,
        "sPercent": 23.839671944504158,
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        "g2G1Ratio": 1.8042307692307693
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      "status": "review-fit"
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    {
      "source": "FR-FCM-Z55E",
      "sample": "G1.fcs",
      "sourceSha256": "9a3f9ad800b5cf1b83a0edb4c38bc0e6e8db9902bbaea7d736b644c1e4238c65",
      "rawEvents": 5154,
      "gatedEvents": 898,
      "selectionRate": 0.1742336049670159,
      "dnaParameter": "PI-A",
      "isBackgroundControl": false,
      "eligibleForReference": false,
      "gate": {
        "fscLow": 9740.2998046875,
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        "sscLow": -4138451.5,
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        "ratioCenter": 0.035647415803363204,
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        "dnaBackgroundLimit": 33795.6015625
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      "gateDescription": "FSC-A/SSC-A central 99%; log(FSC-A/FSC-H) median +/- 1.782; FL2-A (PE-A) > 33795.60",
      "flowapp": null,
      "status": "insufficient-gated-events"
    },
    {
      "source": "FR-FCM-Z55E",
      "sample": "M.fcs",
      "sourceSha256": "3fcdd243108009609694769428814df8b6f691203cc0c0af1f1239ce0131f651",
      "rawEvents": 10000,
      "gatedEvents": 8525,
      "selectionRate": 0.8525,
      "dnaParameter": "PI-A",
      "isBackgroundControl": false,
      "eligibleForReference": true,
      "gate": {
        "fscLow": 398914.6875,
        "fscHigh": 12066585,
        "sscLow": 248397.40625,
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        "ratioCenter": 1.1762421000488883,
        "ratioMad": 0.056695671831439975,
        "ratioRadius": 0.2267826873257599,
        "dnaBackgroundLimit": 33795.6015625
      },
      "gateDescription": "FSC-A/SSC-A central 99%; log(FSC-A/FSC-H) median +/- 0.227; FL2-A (PE-A) > 33795.60",
      "flowapp": {
        "model": "Independent DJF-style",
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        "g2MPercent": 7.088622219869547,
        "g2G1Ratio": 2.0244736842105264
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      "status": "candidate"
    },
    {
      "source": "FR-FCM-Z55E",
      "sample": "S.fcs",
      "sourceSha256": "203baf615cfad76fd4216c087ff423f7e1d69af16ffa8d400d54c241d86fc60a",
      "rawEvents": 76560,
      "gatedEvents": 70077,
      "selectionRate": 0.9153213166144201,
      "dnaParameter": "PI-A",
      "isBackgroundControl": false,
      "eligibleForReference": true,
      "gate": {
        "fscLow": 251156.09375,
        "fscHigh": 6686973,
        "sscLow": 175366.296875,
        "sscHigh": 12794450,
        "ratioCenter": 1.1480950096627625,
        "ratioMad": 0.057569797709106973,
        "ratioRadius": 0.2302791908364279,
        "dnaBackgroundLimit": 33795.6015625
      },
      "gateDescription": "FSC-A/SSC-A central 99%; log(FSC-A/FSC-H) median +/- 0.230; FL2-A (PE-A) > 33795.60",
      "flowapp": {
        "model": "Independent DJF-style",
        "fitR2": 0.9951315686778469,
        "g0G1Percent": 77.22455733190844,
        "sPercent": 14.619848226345022,
        "g2MPercent": 8.155594441746521,
        "g2G1Ratio": 1.9215979381443298
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      "status": "candidate"
    },
    {
      "source": "FR-FCM-Z55E",
      "sample": "unsynchronized.fcs",
      "sourceSha256": "1d5b2671fab4130480c854490a9ba5f1424a94cfe84d92084386bcc45f5ff436",
      "rawEvents": 10000,
      "gatedEvents": 9238,
      "selectionRate": 0.9238,
      "dnaParameter": "PI-A",
      "isBackgroundControl": false,
      "eligibleForReference": true,
      "gate": {
        "fscLow": 243881.59375,
        "fscHigh": 12272468,
        "sscLow": 153694.203125,
        "sscHigh": 21838908,
        "ratioCenter": 1.0963263272546482,
        "ratioMad": 0.10525386809515691,
        "ratioRadius": 0.42101547238062764,
        "dnaBackgroundLimit": 33795.6015625
      },
      "gateDescription": "FSC-A/SSC-A central 99%; log(FSC-A/FSC-H) median +/- 0.421; FL2-A (PE-A) > 33795.60",
      "flowapp": {
        "model": "Independent DJF-style",
        "fitR2": 0.8572782148432254,
        "g0G1Percent": 50.264277390549495,
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        "g2MPercent": 16.829938112073776,
        "g2G1Ratio": 2.1370408163265306
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      "status": "candidate"
    }
  ],
  "limitation": "This is a reproducible preflight and common-input export. It is not a FlowJo or ModFit result oracle until independently fitted values are entered into the template."
}